Additionally, the partial fimbrial gene cluster which was transferred with the insertion is known to be plasmid-encoded [ 46].
Irradiated B6D2F1 mice, which were transferred with the same amount of syngenic (B6D2F1) cells served as controls.
Wild type A. tumefaciens strains are known to harbour cytokinin synthesis genes, which are transferred with the T-DNA to the plant cell forcing transformed cells to produce cytokinins.
We previously demonstrated that Vβ6+ T cells which are transferred with other splenic lymphocytes from the donor strain B10.D2 into tumour-bearing DBA/2 mice are able to break the anti-tumour tolerance (Schirrmacher et al, 2000) and to infiltrate the metastasised organs (Schirrmacher et al, 1995).
Each plant was infested with 32 wingless aphids (4 per leaf), which were transferred to leaves with a fine paintbrush.
In an alternative attempt to achieve better information about the hmC content, an enzymatic approach was taken in which UDP-[3H]glucose was used as a marker, which was transferred onto hmC with the help of a specific glucosyltransferase.
We included information on incapacity benefits, which was transferred to persons with at least 50% permanently reduced ability to work [ 19].
After evaporation of THF, the remaining complexes were treated with 57% HI (0.3 mL) to produce [11C]CH3I, which was transferred under N2 gas with heating into a reaction vessel containing anhydrous DMF (0.3 mL), 3 (1.0 mg), and NaOH (0.01 mL, 0.5 M) cooled to −15°C to −20°C.
The samples were incubated at 100°C for 5 minutes and loaded onto a 10% SDS polyacrylamide gel, which was transferred to nitrocellulose and probed with α-HA antibody.
Oesophageal cancer cells were suspended in RPMI-1640 medium at 5 × 10 ml−1 and added to inserts, which were transferred to wells containing RPMI-1640 medium with 10% FBS.
