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Correlation of inter-sample values requires data normalization, which can be accomplished by various means, the most common of which is normalization to internal, stably expressed, reference genes.
One possible reason is overestimation of mES expression levels by the original method (Li et al., 2011), which is normalization by putative stably expressed endogenous features.
These findings satisfy the primary goal of diabetes management as set by the IDF guidelines, which is normalization of the glycaemic status with HbA1c, FPG and PPG as markers of disease control [ 22].
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The ESTs of ribosomal and mitochondrial proteins, which are normally removed during normalization or preprocessing of ESTs, were not removed in this study.
Arrays were normalized using Subset quantile Within Array Normalization (SWAN) through minfi, which is a normalization method designed specifically to address the technical and biological variability in the Methylation450K arrays due to the type I and type II probes.
Thus, the second step is required which is the normalization of the planes in the same interval so they can be fused.
This normalization, which is comparable to normalizations used elsewhere [71], corrected for normal fluctuations in anesthetic depth between measurements and inter-animal variability within a group.
Similarly, it would be of interest to incorporate the dynamic effects of vascular normalization on macromolecular permeability, which is reduced following normalization, in order to understand how this influences the response to anti-VEGF therapy.
We normalized the 450K array data using Functional Normalization (FunNorm), a novel between-array normalization method, which is based on quantile normalization and uses control probes to act as surrogates for unwanted variation [ 19, 20].
Forth layer: In this layer which is known as normalization layer, each neuron computes the normalized firing strength of a given rule after receiving inputs from all neurons in the rule layer.
We used quantile normalization, which is the most widely used normalization method for genetic studies [ 22].
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