By using poly-lysine for crosslinking silica beads, cell membranes are isolated as cross-linked membrane layers which are disadvantage for performing functional studies [34].
However, their high Ms required a high reaction temperature and expensive high-boiling point toxic solvents, which are disadvantages for their biological applicability.
"Most surfaces you pee into, such as porcelain, are hydrophilic, which is a disadvantage.
TEGDME reduces the reaction rate, which is a disadvantage for its use as a solvent.
Most bacterial laccases studied so far are located intracellularly, which is a disadvantage for micropollutant degradation (Sharma et al. 2007).
Existing OBSs have relatively short recording period, usually a few months, which is a disadvantage for continuous monitoring.
However the current density distributions are less uniform in MOLB-type SOFC, which is a disadvantage in this type SOFC.
Also, Neuro computing and evolutionary computation usually need a lot of computational time, which is the disadvantage of the implementation of soft computing (Dote and Ovaska 2001).
Due to its location, the temperature measurement suffers from long dead times, which is a disadvantage for time-sensitive control strategies.
However, this new PS-targeting probe had a higher uptake in the liver, which is a disadvantage for imaging of liver and myocardial apoptosis [56].
As a result, fine-scale transient models require large computational time, which is a disadvantage for state estimation, forward prediction or model inversion.
