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Even though interesting, this issue posed some remarkable challenges, one of which is constraint handling.
Models are M8A, ω following a β distribution discretized into 10 categories of similar frequency (0 < ω1 10 < 1) plus an additional category of ωad = 1, accounting for neutral sites; M8 differs from M8A only by the additional category of ω which is constraint to be superior to 1 (ωad > 1), to account for sites under positive selection.
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The classical method used for metabolic flux analysis is Flux Balance Analysis (FBA), which is constraint-based and relies on the assumption of steady-state metabolite concentrations throughout the network.
In my view, that adds up to a sizeable interest-free subsidy, which is a constraint to scale.
This addresses the problem of limited access to clinical sample material, which is a constraint of many researchers and clinicians [ 30].
Here, we propose another type of power constraints, which is a bound constraint.
The in silico simulations were performed by flux balance analysis (FBA), which is a constraint-based flux analysis method (Orth et al. 2010; Toya and Shimizu 2013).
Another difficulty is the terminal state constraint, which is a sample-wise constraint.
This is due to the eigenvalue constraint in Equation 5, which is a nonlinear constraint and thus yields the nonlinear bounded space ℱ n − 1.
There are additional constraints that the pulse should satisfy, the most important of which is a bandwidth constraint.
The available amount of substrates is a further constraint which is implemented as a hard constraint for the input variables.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com