We then entered into a coalition agreement, and it's the coalition agreement that is binding upon us and which I'm trying to honour".
When we were about to sign the above-mentioned articles, which were to be binding upon us, our heirs, etc., for fifty years, Mr. Brockden, the scrivener, said to us, "You are young men, but it is scarcely probable that any of you will live to see the expiration of the term fix'd in the instrument".
The first stated, "This agreement shall be binding upon the customer upon the earlier of (a) the date upon which the first transaction is processed by WorldPay … for the customer, or (b) the execution of this agreement by the customer".
Pak1, Pak2 and Pak3 encompass the Group 1 Pak kinases, and all three members contain an auto-inhibitory domain, which is released upon binding of Cdc42/Rac1 to the CRIB domain, and a β-PIX binding site, an interaction previously shown to be important for Pak activation [19].
When a DSB occurs, the Ku heterodimer (Ku80/Ku70) first binds to the broken ends by using Ku80 and then recruits the DNA-PKcs, which is activated upon binding to Artemis nuclease, and the repair process is completed by XRCC4-DNA lIVase IV [ 39, 41].
The proteolysis of the toxin is mediated by a conserved internal Cys protease domain (CPD), which is activated upon the binding of the small molecule inositol polyphosphate (IP6).
In the basal state of arrestin-3, T1A peptide, which includes the N-terminal extension not resolved in crystal and β-strands I and II (Zhan et al., 2014) (Figs. 1 and 4), is partially shielded by the C-tail (Fig. 2C), which is released upon receptor binding (Zhuo et al., 2014), ensuring better exposure of this element.
The C-terminal region of E2F1 is required for its transcriptional activation function, which is inhibited upon the binding of RB [22], [23].
In addition, miRNA sponges can be used to monitor miRNA activity when expressed together in the same transcript with a fluorescent protein gene, which is downregulated upon the binding of the miRNA (Ebert et al., 2007).
PTMs in the hinge region could affect steric properties, while changes to the C-terminal end, which is exposed upon calcium binding, could directly affect the proteins interacting capabilities.
