Another type of consistency violation in the same model relates to the annotation of a reaction which is annotated with a class of physical objects.
Again, almost all of these proteins are of unknown function except PAB2241, which is annotated as RNase P, but this annotation seems arbitrary as it does not show significant sequence similarity to known RNases.
In Hawthorne's "Strange Case of Dr. Jekyll and Mr. Hyde," for example, one clue to Hyde's identity is a "pious work" in Dr. Jekyll's library which is annotated with "startling blasphemies".
Now it has branched out to other documents, like "The Great Gatsby," the manifesto of the Los Angeles police officer-turned-killer Christopher Dorner and Archibald MacLeish's poem "Ars Poetica," which is annotated in images.
A full-length cDNA (AK102748) for ENG1 of rice, which is annotated to encode endo-glucanase, was inserted into the multi-cloning site of pBUH3.
One of the genes specifically down-regulated in Eurosis 3 h-treated roots was LOC_Os06g08480, which is annotated as the CHD3-type chromatin-remodelling factor PICKLE.
A full-length cDNA (AK108835) for EXG1 of rice (Oryza sativa), which is annotated to encode exo-glucanase in a rice full-length cDNA database (KOME database:http://cdna01.dna.affrc.go.jp/cDNA/), was inserted into the multi-cloning site of pBUH4.
An entire coding sequence of a full-length cDNA (AK070962) for BEG1 of rice, which is annotated to encode β-glucosidase, was amplified with BEG1-specific primers (BEG1-F1cut: 5'-CACCATGGCAGCTGCAGGGGAA-3' and BEG1-R1: 5'-GGTGAGCCGTGCTCAAGGAG-3'), and cloned into pENTR (Invitrogen).
RiOG_1497 shares limited similarity with RiOG_1439, which is annotated as a HP and found only in the R. bellii genomes and the chromosomal genome of R. felis.
It is currently unclear whether the aforementioned putative TonB-dependent receptor META2_0097, which is annotated as an iron transporter, might also contribute to cobalt transport across the outer membrane in M. extorquens AM1.
The product of hp1019, which is annotated as a serine protease in the respective GenBank file, seems to be a homologue to heat shock protein HtrA from Escherichia coli.
