This indicates that over a 1000-fold inprovementhen the binding affinity was achieved by dextran conjugation, which is a similar degree of improvement that was observed with the monoclonal anti-AQP4 antibody.
Compared with negative control, in the presence of AtSAE1a-SAE2, andCE1AtSUMO1tSUMOylationylatiof of DnSIZ1 was detected, indicating that DnSIZ1 can be sumoylated by E2 SUMO conjugation enzyme, which is a characteristic feature of SUMO E3 ligase (Fig. 10a).
First, MU depletes cellular UDP-GlcUA, which is a precursor of HA, because of massive glucuronic acid conjugation to MU (Kakizaki et al, 2004; Kultti et al, 2009).
Apparently all of nature, as the British scholar William Ralph Inge observes, "is a conjugation of the verb to eat".
The conjugation of a sugar moiety to a substrate is called glycosylation, which is a process that contributes to the synthesis of glucidic polymers, glycoproteins and glycolipids.
Treatment of cells with camptothecin results in conjugation of Top-1 with SUMO-1, which is a possible repair response to topoisomerase-mediated DNA damage [ 42].
Ubiquitin conjugation sites of protein (Ubiquitylation), which is an essential post-translational modification, is a sequential process that involves a group of enzymes known as E1 (activating enzyme), E2 (conjugating enzyme) and E3 (ubiquitin ligase).
HIC analysis of the final product highlights the facile analytics, which are a major benefit of site-specific conjugation approaches as compared to global conjugation strategies.
WRN interacts with Ubc9, which is required for conjugation of SUMO-1 to the N-terminal fragment (amino acids 272 514) of WRN (Kawabe et al 2000).
The data verified that SENP3 abrogates the interaction between RNF4 and Sp1, which is apparently a consequence of the de-conjugation of SUMO2/3 from Sp1.
