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The affinities of the A1 and G12 moAbs for these peptides were determined by a competitive assay in which immobilized gliadin was challenged with peptides as competitors.
The affinity of the G12 moAb for different hexapeptide variants was determined in a competitive assay in which immobilized gliadin was challenged with QPQLPY-derivative peptides as soluble competitors (Figure 4A).
In control experiments in which immobilized cilia were scanned sequentially, a similar resting [Ca2+] was determined (167 nM, Figure 4 figure supplement 1C, and see below).
This interpretation was confirmed by the reciprocal experiment in which immobilized His-tagged Mcl-1 robustly pulled down GST-tagged VDAC1 and 3 but not VDAC2.
This is in contrast to the current study in which immobilized VDAC1 and 3 is readily pulled down by full-length Mcl-1 from cell lysates.
We developed hepatocyte growth factor (HGF /heparin-immobilized collagen film by adding HGF /heparin-immobilized film, whicollagenilized heparin by chemical cross-linking.
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Spotted arrays currently being used can be divided into two groups based on the nature of immobilized DNA used: one in which the immobilized DNA is comprised of both sense and antisense strands (usually PCR product) and the other where the immobilized DNA is single stranded consisting of only the sense strands (usually, chemically synthesized oligonucleotides).
Modeled on the Prometheus, it recalls Aeschylus's tragedy both in its form, in which the immobilized hero receives a sequence of visitors, and in its theme, in which there is a resurgence of the hero's spirit under stress.
Both genosensors rely on the hybridization of the oligonucleotide target with its complementary probe, which is immobilized on positively charged polylysine modified screen-printed carbon electrodes (SPCEs), through electrostatic interactions.
This process, which uses immobilized glucose isomerase, takes place in a packed-bed reactor that consists of microporous particles with a range of pore sizes, characterized by a pore size distribution.
The resonant frequency shifted due to the specific binding of the PSA Ag to its Ab which is immobilized via calixcrown self-assembled monolayers on an Au surface deposited on a nanomechanical PZT cantilever.
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CEO of Professional Science Editing for Scientists @ prosciediting.com