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Each amplicon (or BAC of interest) in a 384-wells plate was identified by coordinates which identified positive line and column pools in a matrix.
Thus, albeit the need for cloning, a medium-throughput protocol for gain-of-function screen of selected microRNAs could be established, which identified positive effects for miR-17 on CHO growth performance (Jadhav et al., 2012).
Our results for MEHP and endometriosis do not agree with findings from the three case control studies on this subject, all which identified positive associations between markers of DEHP exposure and risk of endometriosis.
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There has only been a single small-scale study comparing DEFA1A3 copy number with HNP1-3 expression, which identified a positive correlation [ 19].
We found no effect of interventions on cardiorespiratory fitness at 12 months across four trials, by contrast with the systematic review by Hillsdon and colleagues, which identified a positive effect on fitness at a minimum of six month follow-up.
The results are comparable to a Cochrane review of interventions in the general population using self-report physical measures which identified a positive moderate effect (SMD 0.28 95% CI 0.15 to 0.41, I 83.5%).
Primer pairs were used for PCR screening of 18,432 BAC clones, corresponding to 6 genome equivalents, which identified 1,833 positive BAC clones, corresponding to 5 clones per marker on average.
An exception is the study carried out in the Postmenopausal Estrogen/Progestin Intervention Trial [ 33], which identified a positive association between percentage density and estrone, estradiol and free estradiol levels in postmenopausal women.
In Latin America the association between the improvement of health conditions and the implementation of PHC has been reported in several studies, which have identified positive impacts on infant mortality rate (IMR), post-neonatal mortality and under-5 morateity rate [ 15- 19].
This allowed design of mouse primers which identified a single positive clone in a bacterial artificial chromosome (BAC) library of mouse genomic DNA.
The genes induced by phosphate limitation and the molecular mechanism by which the genetically identified positive \ pho7^+)\) and negative \((csk1^+)\) regulators function are not known.
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