The frequency of subclones which have deleted the selectable markers ranged from 60%to100%0% depending on the targeted locus (data not shown).
The GAL_059 strain, which had deleted competing pathways (adhE, ldhA, paaFGH, frdABCD, pta) and overexpressed the producing pathway (atoB, lpd-G1060A, aceEF), was transformed with pCDF-BuOH containing heterologous n-butanol producing enzymes (crt, hbd, ter, adhE2) to yield the GAL_061 strain.
This is consistent with the genome content of clade II strains, which have deleted virtually all large surface proteins annotated as adhesins and the two exopolysaccharide clusters present in L. reuteri strains from rodents (supplementary fig. S1, Supplementary Material online; Frese et al. 2011).
The Russos contribute a commentary track to the DVD and Blu-ray, which also has deleted scenes and featurettes.
A pastor at a Florida megachurch, he says America has "deleted God," which has resulted in the "kinds of problems" like legalized abortion and same-sex marriage.
The first government victory shot down amendment 8, which would have deleted the suicide clause in the legislation.
After screening transformants on FOA, six FOAr colonies were identified, of which four had deleted URA3.
We prepared the protein fraction from the cytoplasm, periplasm and OMVs of a strain of B. thetaiotaomicron from which we had deleted the minpp gene.
The current analysis, in which we have deleted Vegfa in VECs during development, also indicates that autocrine Vegfa is important at developmental stages.
This plasmid is a 1.7 kb derivative of pUC19 from which we have deleted most non-essential DNA sequences to remove insertion targets within the vector sequence.
