High throughput 454 sequencing which generates longer reads has been widely used in many transcriptome sequencing studies in non-model species previously [ 48, 49].
To assemble the plastomes sequenced using the MiSeq, which generates longer reads than HiSeq 2000, CLC Genomics Workbench 6.0 and Newbler 2.6 (http://454.com/products/analysis-software, last accessed April 6 , 2015 were used.
In cis but not in trans over-expression of the antisense KAT5 gene promoted the usage of distal polyA (pA) site in sense gene RNASEH2C, which generated longer 3′ untranslated region (3′UTR) and produced less protein, accompanying with slowed cell growth.
Other protocols (e.g. RNAseq), which generate longer tags, can overcome this limitation but they introduce new problems, the most important of which is multiple tags per transcript and a bias towards highly expressed long transcripts [ 55].
With application of the new generation of 454-sequencing (GS FLX Titanium), which generates more and longer reads, one would expect to get longer contigs and more contigs covering the whole of the gene coding sequence [ 51].
In contrast, the 454 pyrosequencing platform, which generates fewer but longer reads, is advantageous in shotgun metagenomics as these longer reads enable better functional assignments of sequenced gene fragments.
A typical example is the deletion of the glutamine at p4 in the DQ2-Glia-α1 epitope which generates a peptide that no longer binds to HLA-DQ2, presumably due to defective docking of the anchor residues into their respective pockets in the HLA-DQ2 molecule.
The oxidation of pyritic mining waste is a self-perpetuating corrosive process which generates acid mine drainage (AMD) effluent for centuries or longer.
The new mobile phone concept apart from harnessing clean energy from sugary drinks like Coke or Pepsi, which generates water and oxygen, also works about four times longer than contemporary lithium batteries on a single charge.
The longer of the two ORF is expressed as a polyprotein which generates a number of variable length peptides of unknown function during processing.
The assembly generates longer sequences, which gives a better chance of association of sequences with the proteins.
