Gene expression profiling data from ZHBTc4 ESCs, which express Oct4 from a doxycycline-repressible transgene (Sharov et al., 2008), was combined with two different sets of Oct4 ChIP data (Chen et al., 2008b; Kim et al., 2008).
Wild-type, vac17Δ, pep12-60 tsf, and vac17Δ pep12-60 tsf cells, which express Whi5-3xGFP from its endogenous locus, were incubated at 24°C overnight, and then sifted to 37°C for 0 or 4 hr.
FM4-64 labeled vac17Δ cells which express Vph1-CFP from its endogenous locus, and Fab1-3xGFP expressed from a CEN plasmid with its endogenous promoter were used.
FM4-64 labeled vac17Δ cells which express Vph1-CFP from its endogenous locus, and GFP-Sch9-2D3E expressed from a CEN plasmid with its endogenous promoter were used.
Two independent T. brucei 221VP117 clones were generated which express two VSGs from the same VSG expression site.
E3 cells, which express human MUC1 from its own promoter, were generated from 410.4 cells as described by Lalani et al (1991).
Wild-type, vac17Δ, tor1Δ, and vac17Δ tor1Δ cells which express Vph1-GFP from its endogenous locus, were pulse labeled with FM4-64.
Indicated plasmids were transformed into wild-type and vac17Δ strains, which express Vph1-CFP from its endogenous locus: pRS416 GFP-SCH9, pRS416 Tor1-3xGFpRS4160), or pRS416 KOG1-3xGFpRS416
In X ESxr bO mice, which express Zfy exclusively from the Zfy2/1 fusion gene, there is reduced MI apoptosis in response to the unpaired X ESxr b chromosome.
(A ) Wild-type and vac17Δ cells which express Vph1-GFP from its endogenous locus, were pulse labeled with the vacuole specific dye FM4-64.
GRIM.miRNAoff vectors can be permanently switched on by transformation into EL350 E. coli cells, which express Cre recombinase from the arabinose-inducible promoter [ 52].
