Detection is based on the use of sequence-specific probes labeled with intrinsically fluorescent europium or terbium chelates and complementary quencher probes, which enable sensitive, homogeneous closed-tube assays without the risk of carryover contamination.
It is likely in the future that there will be overlap in the functional outcomes that are utilized, which enable sensitive monitoring of functional recovery and determination of the efficacy of interventional strategies.
Air has a Hounsfield coefficient of −1000 on CT, which enables the sensitive and specific detection of even minute quantities of intracranial air; 0.5 mL of air can be visualized with CT in contrast to 2 mL, which is the minimum that can be seen with plain films [9].
However, to identify biomarkers that are reproducible and clinically applicable, development of a novel technology, which enables rapid, sensitive, and quantitative analysis using hundreds of clinical specimens, has been eagerly awaited.
A keynote by Piero Carninci (RIKEN) described studies using DeepCAGE [ 29], which enables highly sensitive detection of transcription start sites via NGS sequencing of 5' capped mRNAs.
In this study, this was combined with on-chip immunoaffinity chromatography and ion-exchange chromatography, which enables simultaneous sensitive detection of various PSA glycoforms.
The non linear reference range for blood-derived proteins in CSF forms the base for Reibergrams [ 2, 3, 5], which enables the sensitive and quantitative detection of additional intrathecal synthesis [ 3].
Therefore, we have developed a new method, Competitive Amplification of Differentially Melting Amplicons (CADMA), which enables very sensitive mutation detection regardless of the melting properties of the mutations to be detected [ 9].
Tomography employing a Talbot interferometer, which enables highly sensitive observation based on X-ray differential phase contrast, can reveal the refractive index decrement [ 15], a value that corresponds approximately to electron density and (for materials consisting of light elements) mass density [ 16].
In this work, we demonstrate a novel estrogenic receptor fragment-based homogeneous fluorescent assay which enables rapid and sensitive detection of 17β-estradiol (E2) and other highly potent estrogens.
