Exact(1)
The trimmed ganglia were digested with collagenase type IV (2.67 mg/mL, Sigma, USA) and trypsin type I (1 mg/mL, Sigma, USA) at 37°C for about 30 min. Single cells were dissociated mechanically with a series of fire-polished Pasteur pipettes, plated on glass slides which covered with Poly-D-Lysine (PDL), then placed into dishes.
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The primary federal law that comes into play is the Americans With Disabilities Act, which covers employers with 15 or more employees.
We want to get rid of the innumerable museums which cover it with innumerable cemeteries.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

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CEO of Professional Science Editing for Scientists @ prosciediting.com