Many bacterial species lose essential biosynthetic pathways, disabling them from living autonomously, which may explain why more than 90% of bacteria cannot be cultivated in the absence of a community environment (Costerton et al., 1994; Johnson et al., 2012).
Some other amino acids may be needed to maintain the metabolic pathway in C. cellulovorans, because the bacterium cannot be cultivated in media without yeast extract, which has glutamate (Sleat et al. 1984).
Lymphocytes are short-lived, however, and cannot be cultivated satisfactorily.
And since white truffles cannot be cultivated, well-trained dogs like Diana are valuable resources.
The trees produce fruit only in undisturbed habitats and cannot be cultivated in pure stands.
Shrimp farmers in Bangladesh mainly cultivate Indian Tiger Shrimp (Peneaus monodon), a species that cannot be cultivated in fresh water (Sarwar 2005).
They are hard to find (most truffle hunters use trained dogs to sniff them out from where they lie buried next to tree roots) and cannot be cultivated.
Other microbial groups, including the majority of species in many ecosystems, cannot be cultivated with current methods [12].
M. leprae is an obligate intracellular parasite that cannot be cultivated in vitro.
The majority of anaerobic bacteria present in our digestive tract cannot be cultivated on existing media.
