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For example, a BAC clone with a size of 100 kb requires about 600 shotgun clones which are sequenced from both ends to be sufficiently covered (7 runs on a 3730xl sequencer).
It consists of a series of correspondence matrices which are sequenced to reflect a typical path in the product development process such that outputs of a matrix are inputs in a succeeding matrix (Fogliatto et al. 2003).
Multilocus sequence typing (MLST) uses neutrally selected housekeeping genes (7 ), which are sequenced with automated equipment (8 ).
Enzyme-specific adapters tagged with different barcodes are then ligated to the restriction fragments and the restricted fragments which are sequenced using Illumina short-read sequencing.
Unlike the reads for HuRef data, which are sequenced with the Sanger-based whole-genome shotgun sequencing and therefore are very long (each segment is thousands of base pairs long including both homozygous and heterozygous sites), here we consider the reads generated by the HTS technology (Wheeler et al., 2008).
Digital gene expression: tag profiling (here called Tag-Seq, also called DGE:tag profiling), specifically, uses a restriction enzyme (NlaIII) with a common recognition site (CATG) to create "tags" from all polyadenylated transcripts, which are sequenced in an known orientation relative to the poly-A tail giving strand-specific information (for details, see Materials and Methods).
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Glycerol stocks were generated by selecting approximately 15,000 clones from each library which were sequenced using ABI3730 sequencers following standard protocols for sequencing analysis.
Each sample was used for the construction of RNAseq libraries, which were sequenced using an Illumina Genome Analyzer IIx sequencer.
Seven hundred twenty colonies were screened, 502 of which were sequenced on an ABI PRISM 3730 automated DNA sequencer (Applied Biosystems).
It was greater than the sum of its songs, which were sequenced to provide a sort of cumulative dramatic arc.
Dr. Moran, who studies genomic evolution, made the discovery while searching through the pea aphid genome, which was sequenced last year.
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