The present study was designed to examine the effects of the above-mentioned compound on VacA-induced vacuolation of HeLa cells, which was quantitated using the neutral red uptake assay.
The effluent and water washes contain neutral oligosaccharides which were quantitated by scintillation counting.
MDA products from both individual cells and the bulk enrichment were used to create sequencing libraries, which were quantitated using digital PCR [36] and sequenced.
Each loop was weighed and incubated with formamide for 24 hr to elute the amount of EB absorbed, which was quantitated spectrophotometrically at 560 nm.
Although some cell death was observed at 24 hours post-transfection, it was most evident at 48 hours post-transfection, which was quantitated by the Trypan blue exclusion test of cell viability.
Native CYA, AMN, AMD and MEL were quantitated against their corresponding C-labelled recovery standards which were quantitated against their corresponding N,C-labelled performance standards.
Cell lines were selected with increasing doses of methotrexate, sub-cloned and expanded to a stable clone producing the desired IgM, which was quantitated by ELISA and tested for potency.
The relative amount of spliced-XBP1 mRNA in five MM samples (#2, #4, #5, #8 and #9), which was quantitated by real-time PCR method, decreased after 6 h exposure to toyocamycin.
All runs were performed on a Rotor-Gene 6000 (Qiagen) and comprised at least two no-template controls as well as duplicates of serial dilutions of positive controls, which were quantitated using the Quant-iT PicoGreen assay (Invitrogen, Paisley, UK), corresponding to c. 10, 10, 10 and 10 genomic equivalents (GE)/μL.
MDA, the end product of lipid peroxidation, was analyzed according to the method of Buege and Aust in 1978[ 44] which based on the reaction of MDA with thiobarbituric acid (TBA) to form MDA-TBA complex, a red chromophore, which can be quantitated spectro-photometrically according to this method.
