Addition of pPGK-nlsCRE to the transfection, alone or in combination with pKUb-SB11 reduced puromycin-resistant colony counts to levels significantly lower than that observed for pKUb-SB11 or pCMV-β, which alone result in TnT and non-transpositional transgenesis with an intact PTK gene expression cassette, respectively.
Counting the respiratory rate or sending the patient out to be weighed, however, require somewhat more effort which alone might result in a failure of documentation.
Crossbreeding heterozygous Cd2ap knockout mice with mice heterozygous (knockout) for synaptopodin or Fyn, which alone did not result in clinical kidney pathology, resulted in spontaneous proteinuria and FSGS-like glomerular damage supporting a role for CD2AP haploinsufficiency [ 60].
However, in both cases the RT step remains which alone can still result in erroneous high frequency variants, although only at relatively few genome positions.
The most important type of resource is training, which alone gives nearly as good results as the union of all types of resources.
An exception to this was OPM2 cells, in which doxorubicin alone resulted in 54.7% positive TUNEL staining.
Genome-wide association studies targeting common variants which, alone, influence susceptibility have produced mixed results [ 1- 5].
In addition, accelerated bone turnover has in both traumatic and estrogen deficiency models (ovariectomy (OVX)) been shown to augment articular cartilage erosion [ 7, 11, 12, 44, 45], in which increased bone resorption alone results in increased articular cartilage damage [ 10, 37, 46].
The substance of such 'worries' is difficult to evaluate from the quantitative findings alone, results of which are limited by the specific questions asked.
This was also apparent in the absence of adjuvant, in which immunization with mBSA alone resulted in low but significant antigen-specific production of total IgG and IgG1, which was not increased in the presence of 1,000 U IFN-α (see Additional file 1: Figure S1).
