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To test for the role of purifying selection for genes that did not show evidence of positive selection, we extracted parameter estimates from model M1a in PAML (Yang 2007), which allows a proportion of sites to be neutrally evolving (i.e., ω = 1 ), and the remaining sites to be subject to purifying selection (i.e., ω < 1 ).

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The tight packing is needed to achieve a high photopigment density, which allows a large proportion of the light photons that reach the photoreceptor to be absorbed.

In an appropriate buffer, organic fluorophores can bleach reversibly ("blinking"), which allows a small, random proportion of the fluors in a field to be active at one time.

Another important component of model accuracy is agreement between predicted probabilities and observed proportions, known as calibration, which allows a model's prognostic ability to be evaluated [ 5].

But a credit event probably would occur if all bondholders were forced into the switch.Some sovereign bonds have "collective action clauses" which allow a government to impose a deal on all creditors if a certain proportion of them agree.

Participants were recruited to provide a sample size of 1,000, which allowed a precision of 2%3%% when calculating a 95% confidence interval of a dichotomous variable with a base proportion ranging from 10%to50%0%.

A fixed number of 100 embryos were collected over a 1h period at room temperature and left to develop for 4h, which allows having a high proportion of stage 9/10 embryos.

To ensure a more representative picture of temporal trends in the proportion of ORVs, a mixed effect logistic regression modeling approach (26, 27 ) was used, which allows modeling of binomial proportions, i.e., a numerator and a denominator as a function of time, where the coefficients of this function are allowed to vary for each country around a mean value, combining data from all countries.

To analyze the methylation data, we used the Kismeth software (http://katahdin.mssm.edu/kismeth/revpage.pl), which allows visualization of the proportion of clones (epialleles) showing methylation at every particular site.

The same samples analyzed for mtDNA were also genotyped for 46 AIM-InDel markers [ 71], which allow the proportion of ancestry accounted for main continental groups to be estimated (Additional file 5).

We found that at least 8 ng of bisulfite-modified, single-stranded DNA in the 25 μl MethyLight reaction mixture was required to generate Ct values for the input reference COL2A1 < 29.3, which allowed the proportion of 1/64 of methylated- P16 to be consistently detected.

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