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The patterns in which AGO proteins and regulated and unregulated reporter transcripts are distributed are indistinguishable.
However, the precise mechanism by which AGO regulates miRNA biogenesis needs to be determined to uncover preferential accumulation of miRNA strands under different conditions.
Guide strand selection is dependent on the first 5' nucleotides and which Ago protein is present in the RISC [ 17].
The first nucleotide does not seem to determine which 5' tRF is directed to which AGO protein, as observed in microRNAs.
The function of AGO, however, has only been studied in eukaryotic systems; and, in the microRNA (miRNA) pathway, the precise mechanism by which AGO proteins mediate translational repression of target messenger RNAs (mRNAs) remains controversial (Eulalio et al, 2008a).
Similar(55)
In D. melanogaster five Argonaute proteins exist, of which AGO-1 (mainly associated with miRNAs) and AGO-2 (mainly with siRNAs) are related to the RISC.
These ex-siRNAs would be produced by a Dicer-independent mechanism in which Ago-1 would not participate in their biogenesis or function, although other M. circinelloides Ago proteins (Ago-2 and/or Ago-3) could be involved.
Small RNAs and their targets bind the Ago-containing RISC complexes, in which the Ago proteins form stable Ago ribonucleoproteins that can be biochemically analyzed [ 53, 57, 58].
The sun, which long ago disappeared in the mist, had set.
The Obama administration, which months ago announced this Tuesday as the deadline, has vigorously disagreed.
"It also corrodes the 'independent' press, which long ago abolished limits to attack Chávez".
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