We fed compound treated larvae egg yolk and after allowing time for its absorption, we performed whole mount stainings using the lipophilic dye oil red o (ORO) to determine whether yolk-derived lipids accumulated in either organ (Figure 5).
To examine whether these yolk androgens had sex-dependent associations on chick digit ratios, their interactions with offspring sex were included in the models.
Subsequently, the question was raised whether diminished yolk precursor uptake by R/O oocytes might be accompanied by an altered level of endophilin III.
However, the inter-specific chimera system used did not permit full development, so there was no evidence from the chick system as to whether the yolk sac-derived cells were retained post-hatch.
To examine whether the yolk-delivered fatty acid analog was incorporated into lipoproteins, we examined a zebrafish model of abetalipoproteinemia (Avraham-Davidi et al., 2012), a disease characterized by the lack of ApoB-containing lipoproteins (β-lipoproteins, such as CM, VLDL and LDL).
We next evaluated whether defects in placenta, yolk sac or vasculature could be responsible of the observed anemia seen in PiT1Δ5/Δ5 embryos.
It is not known whether persistence of the yolk mass impedes gut development in these embryos or reduced gut development is the cause of poor yolk utilization after knock-down of A2ML expression.
We did not collect information on whether participants consumed egg yolk (rich in cholesterol) to further examine the contribution of dietary cholesterol from eggs on type 2 diabetes risk in this study.
To this end, we first examined whether male traits affect the yolk androgen deposition by the female.
However, the source of the increase in microglia, whether primitive macrophages in the yolk sac or fetal liver monocytes, remains to be clarified.
