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It remains unclear whether the inserted DNA itself was CpG rich or whether CpG sequences were accumulated after the insertion event.
Dr. Smith said he was not sure whether the inserted genome destroyed the host genome or just made the cell divide, assigning the two genomes to different daughter cells.
Although the researchers don't know whether the inserted viral sequences have a function, "they suspect they are helpful to the animals otherwise they wouldn't have endured through millions of years of evolution".
An important issue is whether the inserted Ig spacer domains are inert and only act as spacers, or could in themselves be having an effect.
In addition, the genomic site of integration may be an important parameter that governs whether the inserted promoters can affect or be affected by the neighboring genes.
In particular, it is unclear whether the inserted sequence directly participates in receptor binding as both RPTP α and RPTP β are recognized by the m2 type of vacA alleles [ 25].
Similar(51)
Therefore, to determine whether Cre-recombinase could utilize the inserted LoxP sites to delete Wwox Exon 1, we generated mice combining the Wwoxflox allele with the Cre-recombinase gene under the control of the adenovirus EIIA promoter.
We asked whether the intragenomic presence of the inserted miRNA in HIV-1 prevents viral replication in human cells.
Indels were characterized based upon the length of the inserted or deleted sequence, whether it was intronic, intergenic or exonic.
An initial restriction digest of miniprep DNA prepared from doubly selected transformed target plasmids was used to rapidly determine whether the transposon inserted into the repeat-bearing region of the target plasmid (Figure 1).
To further test whether the mitochondrially inserted Bax promotes Cyt c release, subcellular fractionation experiments were carried out in cells expressing WT or each of A- or E-Bax mutants.
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