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In real-world applications, transactions are changed whether insertion or deletion in a dynamic database.
They did not report whether insertion of miR122 target sequences was enough to attenuate virus replication in HuH7.
This led us to investigate whether insertion of a transposon would interrupt the repeat to a degree that would allow an increase in transcription.
Finally, as our screening method tolerated small local rearrangements within conserved synteny blocks (Materials and Methods), we are able to examine whether insertion of HGT genes is accompanied or not by other local rearrangements.
To determine whether insertion of the Cre expression cassette affected transcription of the surrounding genes, C127 cells were infected with WT or Cre10 33 and expression of K3 and ORF11 which flank the Cre insertion site was analysed by RT-PCR.
To determine whether insertion of a stop codon downstream of the ATG start codon for the UL76 ORF also affects UL77 gene expression in the HCMV- infected cells, we constructed a recombinant HCMV BAC DNA with a flag epitope fused to the N- terminus of the UL77 ORF by reverse selection (Fig. 4).
Similar(45)
A fundamental question in this respect is whether insertions and deletions are governed by similar or different processes and, if so, what these differences are.
The assessment of whether insertions and deletions (if any) can be easily accommodated into the model.
We have not yet tested whether insertions could be created at an even greater distance from the sgRNA site.
To determine whether insertions of MITEs into gene regions are due to chance, we performed a computer simulation as a negative control (see Methods for details).
It was not determined though whether the normal tissues also contained some number of L1 insertions relative to the tumor tissue, and thus whether insertions in the cancer represented an elevated level of retrotransposon mobilization.
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