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Reducing secondary structure by denaturing the mRNA enhances translation [ 15], whereas inserting sequences in the mRNA 5′-UTR that can form secondary structure impairs translation [ 16– 20].
This defect can be mostly rescued by inserting a Ku-binding hairpin at other locations within the mutant tlc1Δ48 RNA, whereas inserting additional Ku-binding hairpins into wild-type TLC1 causes progressive telomere hyper-elongation (Zappulla et al., 2011).
Insertion of too few postsynaptic receptors will lead to an excessive loss of information, whereas inserting too many postsynaptic receptors could increase the local energetic demand beyond that that can be met by the ATP supply from local mitochondria.
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The latter sets deviate from a Poisson distribution (12 species hsp70: chi-square, p<0.0001, Hsp70 in D. melanogaster natural populations: p<0.0001; Set I promoters in D. melanogaster natural populations, p<0.0001), whereas those inserting into the genesets for the 12 species do not (Promoter set I: p = 0.743, Promoter set II: p = 0.279, Promoter set III: p = 0.272).
Spiral inserts are shown to offer a minimal improvement in thermoelectric power production whereas inserts with protruding panels are shown to be the most effective.
First, mutants showing linker insertions within a limited junction region between Domain 1 and the nearby β-helix were no longer able to perform autotransport, whereas inserts juxtaposed to this region did not affect this function.
Un-coated inserts were used for migration assays whereas inserts pre-coated with growth factor reduced Matrigel (40 μl, 1 mg/ml; BD Biosciences) were used for invasion assays.
We performed analysis of all pairs of CISs, using contingency tables that assume one CIS is the predisposing event, whereas inserts in the second CIS are assumed to be subsequent events.
Investigations on slabs with cut-outs revealed that the FRP can be placed only around the edges of the cut-out when retrofitting the slabs whereas, in the situation of inserting cut-outs combined with increased demands of capacity, it is necessary to apply FRP components on most of the soffit of the slab.
Eukaryotes predominately evolve by point mutations (changes in single base-pair in DNA) whereas bacteria often evolve by inserting or deleting large chunks of DNA [ 9- 11].
The low-level interface allows to manipulate the bytecodes itself whereas the high-level interface provides methods for inserting new interfaces, fields, methods or whole code splices.
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