Spiral inserts are shown to offer a minimal improvement in thermoelectric power production whereas inserts with protruding panels are shown to be the most effective.
First, mutants showing linker insertions within a limited junction region between Domain 1 and the nearby β-helix were no longer able to perform autotransport, whereas inserts juxtaposed to this region did not affect this function.
Reducing secondary structure by denaturing the mRNA enhances translation [ 15], whereas inserting sequences in the mRNA 5′-UTR that can form secondary structure impairs translation [ 16– 20].
Un-coated inserts were used for migration assays whereas inserts pre-coated with growth factor reduced Matrigel (40 μl, 1 mg/ml; BD Biosciences) were used for invasion assays.
This defect can be mostly rescued by inserting a Ku-binding hairpin at other locations within the mutant tlc1Δ48 RNA, whereas inserting additional Ku-binding hairpins into wild-type TLC1 causes progressive telomere hyper-elongation (Zappulla et al., 2011).
Insertion of too few postsynaptic receptors will lead to an excessive loss of information, whereas inserting too many postsynaptic receptors could increase the local energetic demand beyond that that can be met by the ATP supply from local mitochondria.
We performed analysis of all pairs of CISs, using contingency tables that assume one CIS is the predisposing event, whereas inserts in the second CIS are assumed to be subsequent events.
The latter sets deviate from a Poisson distribution (12 species hsp70: chi-square, p<0.0001, Hsp70 in D. melanogaster natural populations: p<0.0001; Set I promoters in D. melanogaster natural populations, p<0.0001), whereas those inserting into the genesets for the 12 species do not (Promoter set I: p = 0.743, Promoter set II: p = 0.279, Promoter set III: p = 0.272).
The full-length ZRS transgenic construct was previously described (Lettice et al., 2003), whereas the inserts for the other constructs were generated by PCR and subcloned into the p1230 vector (Yee and Rigby, 1993) containing the lacZ reporter gene and a β-globin minimal promoter.
Whereas the sequence was inserted within a bacteriophage protein-coding sequence in Vibrio parahaemolyticus (chromosome 1), the motif was located in a gene coding for a major facilitator family transporter in the third chromosome of Burkholderia ambifaria (Table S1).
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