Exact(1)
Immunoprecipitations were performed as described in [49], where we probed lysates from HeLa cells (untreated or stimulated for 2 minutes with 5 nM total of purified InlB) looking for binding partners of SEPT9.
Similar(59)
In the third game, at 7 8, came an extended point where we both probed patiently, jockeying for an opening.
We note that we systematically perform controls where we omit probe or primary anti-dig antibody from the protocol, or for selected probes perform hybridization with sense strand riboprobes; none of these controls yield detectable cellular signal, further supporting the specificity of the hybridization results obtained.
Finally, we discuss wave-function tomography experiments, where we directly probe wave functions using a novel experimental technique.
In order to assess the significance of our results, we used a random model where we shuffled the probes of our dataset and compared the distance between the median samples of our two groups to the distribution of 1000 distances of median samples of two random sample groups separated with the same classifier.
Reanalysis of the raw data set was performed using less stringent statistical filters to more fully identify the spectrum of biological functions under NF-κB control, where we identified 74 probe sets.
To avoid distortion, we did not use UMR for normalization for a small portion of probes where we expected chain effect.
These two probe concentrations were chosen on the basis of our previous study with the use of GTP affinity probe, where we observed a 4-fold reduction in the identification of the number of GTP-binding proteins when the concentration of the GTP probe was decreased from 100 to 15 μM.
This gives 20144 different TF-probe pairs where we can classify whether the TF binds to the probe, and then check the classification.
The detection of excitonic state populations in SWNTs is therefore completely masked, except for the 700 nm (1.76 eV) probing wavelength where we most likely see a contribution from the SWNTs and the polymer.
The difference ranges from -12% to +8%, except for the gene GI_4755127 probe 1, where we got 33% (Table 6).
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