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X-ray HSG, nowadays, should not be considered anymore as a "first-line" diagnostic procedure and should be reserved only for settings where the pre-mentioned diagnostic methods are not available or for health systems where indicated for other reasons.
Cells were maintained in phenol-red free DME containing 10% fetal calf serum and 50 mM Hepes or EBSS where indicated for live-cell imaging experiments.
For capillary morphogenesis assays involving taxol or nocodazole, cords first were allowed to form for 3 h and then these agents were added, where indicated, for the final hour of the assay.
These day 5 DC were plated in a 96 well plate at 5,000/well and incubated without or with LPS (10 ng/ml) and without or with Ht31 (100 µM) or Ht31p (100 µM) and PGE2 (1 µM), where indicated, for 24 hrs at 37°C, 5% CO2.
Confidence intervals, where indicated for the difference between the two treatment groups, will be calculated at the 95% level.
The samples were washed and incubated with secondary antibodies and HRP (where indicated) for 2 hr at RT.
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Statistical analysis included, where indicated, test for normality (Shapiro-Wilks), paired and unpaired t tests, one-way analysis of variance (ANOVA) with Tukey multiple comparisons test, two-way ANOVA with Tukey multiple comparisons test, Wilcoxon matched-pairs signed-rank test, Spearman rank correlation, and Mann-Whitney test.
Where indicated RNA for H2BGFP (nuclear localized GFP) (37 ng/µl) was injected into 1 8 cell staged zebrafish embryos enabling 4D imaging of mosaically nuclear labeled zebrafish.
Notes: For the patient-doctor agreement study, n = 122 except for missing responses (maximum 8) or where indicated by asterisk; for patient-patient study n = 141, except for missing responses (maximum 7) or where indicated by asterisk.
Screening for pregnancy, including detailed history and consideration of a pregnancy test where indicated is important for women of childbearing age.
Assessments were made via a questionnaire, a diagnostic instrument and, where indicated, clinical interviews for diagnosing psychotic disorders.
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