Negative controls run in parallel comprised sections where the primary antibody had been omitted or replaced with nonimmune rabbit serum.
Group 3 comprised scenarios where the clean and metal groups are separate lineages, with metal populations being derived from the common metal lineage.
These comprised 4 where isolates were genetically indistinguishable (3 with 0 SNVs) or closely related (9 SNVs) despite patients not having shared time on the ICU and 1 where spa types were discrepant despite isolates only differing by 18 SNVs and patients having shared time on the ICU.
Sequences with identical taxonomic predictions from both homology and composition were assigned to Group I. Group II comprised sequences where the expectation value for the best-matching genome was at least 10 orders of magnitude smaller than the best-matching genome from a different class.
This regimen contained a balance of mouse nutritional ingredients and delivers 4.1 kcal g-1 gross energy, where fat, carbohydrate, protein, and fiber comprised 55, 520, 225, and 45 g kg−1 of the diet, respectively.
This total comprised 38,179 distinct tags where 20,741 (54%) were observed only once (singleton tags).
According to the manufacturer's specification, this diet delivers 4.4 kcal g−1 gross energy, where fat, carbohydrate, protein, and fibre comprised 55, 520, 225, and 45 g kg−1 of the diet, respectively.
According to the manufacturer's specification, the KD delivers 7.8 kcal g−1 gross energy, where fat, carbohydrate, protein, and fibre comprised 700, 0, 128, and 109 g kg−1 of the diet, respectively.
The diet contains a balance of mouse nutritional ingredients and delivers 4.4 kcal/g gross energy, where fat, carbohydrate, protein and fibre comprised 55, 520, 225 and 45 g/kg of the diet respectively.
