Exact(35)
Aligned sequences were edited manually where alignment was poor, by comparison with BLASTP- and PSI-BLAST-derived data.
Open reduction was necessary only in one case, where alignment with manual and closed reduction was not possible to achieve.
Nandakumar [45] applies a binary fixed-length minutiae representation obtained by quantizing the Fourier phase spectrum of a minutia set in a fuzzy commitment scheme, where alignment is achieved through focal point of high curvature regions.
Only regions where alignment was regarded as reliable were used in the input file for MrBayes.
Regions where alignment was doubtful were removed before the phylogenetic analyses were carried out using MUST.
For phylogenetic analyses, gapped sites and regions where alignment ambiguity precluded determination of homology were removed, giving rise to a 7,284 character alignment.
Similar(25)
We compare the results obtained by traditional phylogenetic methods to those inferred by the signature method for two genes: RAG1, which is easily alignable, and 18S RNA, where alignments are often ambiguous for some regions.
For instance, a structurally correct alignment is an alignment where aligned residues play similar role in the 3D structure.
The initial purpose was to speed up database searches, where alignment-based methods were too slow.
Despite the relative success of modern-day sequence alignment algorithms, so-called alignment-free approaches offer a complementary means of determining and expressing similarity, with potential benefits in certain key applications, such as regression analysis of protein structure-function studies, where alignment-base similarity has performed poorly.
This utility performed a pair-wise Smith Waterman overlapped alignment (Smith and Waterman, 1981) between reads pairs, and selected the best quality base where alignments returned mismatching bases.
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