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When we inoculated ASL in vitro (Figure 4, B), PAO1 grew only in the presence of 20 mM glucose (mean OD at 24 h for ASL = 0.231±0.008 vs. mean OD at 24 h for ASL + glucose = 0.643±0.012. p<0.0001).
We observed a biofilm inhibition when we inoculated the strain with a concentration of TQ equal to MIC and 2 × MIC.
When we inoculated macaques with lower doses (data not shown) or when macaques were inoculated on multiple occasions, incubation periods were highly variable.
The reason for using a mixture of cells is that in preliminary experiments, when we inoculated mice with 100% Notch3 shRNA-expressing cells no xenograft growth was observed within 2 months from the date of injection (data not shown), thus no tumour growth was measurable.
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When we inoculate P. yoelii (Py) sporozoites intravenously (IV) into BALB/c mice, the 50% infectious dose (ID50) is often less than 3 sporozoites, indicating that essentially all Py sporozoites in salivary glands are infectious.
In order to find out how the two organisms, both having the ability to utilize the same carbon source, do when cultured together, we inoculated 106cells each of PAO1 and PP9 together in 100 ml Tris BA medium.
TLR KOs but not WT macrophages generated differential and lower levels of TNF-α when infected than when inoculated with unwashed HK-B.
By flow cytometry analysis, we demonstrated that, when inoculated in mice, wild type baculovirus induce IFNγ and IL-12 expression and activation of NK and NKT cells.
The most likely explanation for this finding is that mice used in the current study were 10 to 12 weeks old when tumors were inoculated, and we and others have shown tumors do not take if animals that are older than 8 weeks.
Intraoral pathogens also induce localized inflammatory lesions around the absorbable devices when inadvertently inoculated.
When Simpson inoculated mice with his blood, they became sick and some died.
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