Exact(20)
The silica occurs either as weakly cemented nodules or as hardpan and accumulates as a result of downward translocation (migration) when solubilized during weathering of the soil.
About half of the expressed PGM protein was present in inclusion bodies, but this protein was inactive when solubilized.
Stability can be engineered into IMPs by inserting stabilizing mutations, thereby generating proteins that can be successfully applied to biochemical and structural studies when solubilized in detergent micelles.
Here we apply cellular high-throughput encapsulation, solubilization and screening (CHESS) to the neurotensin receptor 1 (Nto1) to generate a variant that is stable in the apo-state when solubilized in detergents.
Xylanase fused to Zera was biologically active not only when solubilized from PBs but also in its insoluble form.
However, the structural stability parameters of SoPIP2 1 have not been examined neither when solubilized by detergents nor after the protein reconstitution into membranes subsequent to the heterologous expression.
Similar(40)
It was succeeded to prepare a tight and elastic solid by applying very carefully a microwave, when solubilizing the polysaccharide in water.
Figure 1(C) shows that essentially all the histidine tag signal disappeared when Cx26 solubilized in 0.05% DDM (100±0%, n=4) or reconstituted (100±0%, n=6) was digested with TEV protease.
As the A2AR SMALP preserves the annular lipid in close contact with the receptor in the membrane, it was reasoned that A2AR would be more thermostable in a SMALP than when detergent-solubilized by DDM, a widely employed detergent for GPCR solubilization.
While C-terminally tagged QIL1 efficiently co-purified with endogenous MIC60, MIC19, MIC27, and MIC10 when mitochondria were solubilized with 1% digitonin, no specific binding was detected when mitochondria were solubilized with Triton X-100, consistent with an association of QIL1 with the mature MICOS complex.
Therefore, surface complexation may be the major way through which Ag-ENs were solubilized when GSH was used.
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