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Occasionally, however, it can be time-consuming, costly, and labor-intensive when sequencing many influenza viruses.
When sequencing pioneer Craig Venter's team implanted a bacterium with a fully synthesised genome in 2011, they added their names and several famous quotes into the fabricated DNA.
Knowing that many inactivated genes don't result in disease will also be important when sequencing genomes becomes routine in the clinic, MacArthur says.
The standard deviation of the average sequence coverage for the phage genomes using 454 was similar to that observed when sequencing prokaryotic genomes using this technology.
Post-amplification normalization substantially enhanced genome assembly when sequencing effort was <100X, but this effect was smaller at higher coverage (Figure 4b).
When sequencing errors are present, the graph may not be bipartite.
Taken together, Method A provided superior efficiency when sequencing a small number of cells.
The PGM gave very biased coverage when sequencing the extremely AT-rich P. falciparum genome.
No discrepancies were found in the assembly of those two contigs when sequencing the clones.
The difference in cost is especially substantial when sequencing a large genome.
The major obstacle when sequencing DNA pools is the sequencing error rate of the HTS platform.
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