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Eluted RNA was collected and stored at −80°C when not immediately used.
Positive controls were generated by incubation of genomic DNA with SssI, and bisulphite conversion efficiency was calculated from the conversion rate of cytosine to thymidine when not immediately followed by a guanidine as previously described [ 15, 20].
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It is noteworthy that WB samples also degrade over time when not analysed immediately, particularly if not kept refrigerated.
When not used immediately, all lysate supernatants were snap-frozen in liquid nitrogen and stored at −80 °C until use.
Yet when he was not immediately returned to power, Mr. Zelaya repudiated the plan.
And there has to be leadership to ensure that unanimity when it is not immediately forthcoming.
The process by which α-stereocenters of polyketide intermediates are set by modular polyketide synthases (PKSs) when condensation is not immediately followed by reduction is mysterious.
But when he did not immediately vault to the top of the N.H.L. scoring ranks, he was considered an underachiever.
Officials at Stanford, when contacted, were not immediately able to determine if Williams had applied for the Rhodes.
Helmets do prevent some head injuries, and these can be very serious even when they are not immediately fatal.
When she did not immediately respond, he beat her so badly that she wound up in the emergency room.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com