Exact(9)
When Est – Erhard Seminars Training – took off in San Francisco, an offshoot called Exegesis flourished in London.
The possibility that instruments generated more sequencing errors when EST originated from cancer cells does not seem rational.
The apparent overrepresentation of urogenital and nervous systems is not significant when EST library coverage is taken into account.
However, when EST sequences containing SSRs are assessed with the Gene Ontology assigned molecular function, a relative increase of other functions is revealed.
When EST sequences were too short or did not contain the entire CDS, a consensus sequence was deduced using the Bioedit software.
Even when EST genome coverage is not high and, thus, the number of ASPic predicted introns is low, our system should predict genes with an accuracy better than GeneID alone.
Similar(51)
Likewise, in this study, higher polymorphism was also observed when EST-SSR markers included more than 20 bp of SSR length.
In addition, when EST-based markers are identified in a target trait locus, they are generally more effective than other kinds of markers for identifying genes that affect the relevant traits [ 61, 62].
When EST-SSR primers designed from Arabidopsis were used against other species, again low transferability rates were found, being the best positive cases found in Physcomitrella, Pinus and rice with amplification rates of 1.04%, 1.20% and 1.90%.
In these cases, we only aligned the corresponding sequences starting from the first ESTs verified site when ESTs were available, or from the site where the alignment started to be unambiguous, when ESTs were not available.
In microarray data, the number of detected genes was lower than that in EST data, even when ESTs have not been sampled deeply enough.
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