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Therapeutically adverse characteristics were reversible, as resistant, dormant cells became sensitive to treatment and started proliferating when dissociated from the in vivo environment.
Our efforts to understand the bioimmunological role for CRP have focused on carefully examining its higher order structure (i.e., secondary, tertiary, and quaternary) and the changes to subunit structure when dissociated from the CRP pentameric disk.
Most normal epithelial cells die when dissociated from the epithelium.
The force of cell contraction in a dispase lifted control sheet compresses the cell so that the cell height is greater than the cell's lateral dimension, indicating the cells have columnar morphology when dissociated from the cell plating surface.
Indeed, when dissociated from coincident NaV modulation, we found that AIS shortening is associated with a decrease in neuronal excitability on measures of multiple, but not single, AP firing.
The function of LSD1 is known to be context-dependent (Lee et al., 2005; Shi et al., 2005) and under certain circumstances and when dissociated from HDAC1/2 and CoREST can function as a co-activator by the demethylation of repressive H3K9 marks.
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When dissociated striatal neurons from E14.5 Sprague Dawley rats were mixed with those from E18.5 rats, combined cultures from these two gestational periods yielded surviving cholinergic interneurons and representative populations of the other interneuron types at 5 weeks in vitro.
When dissociated cultures of pancreatic cells from neonatal mice (P0 P2) were co-stained for NPY and insulin, a population of cells was identified that contained punctate NPY-immunoreactivity (NPY-ir) as expected for a transmitter located in the regulated secretory pathway (Figure 1A).
Cy5 and FRET signals disappeared when Cy5-V dissociated from the E-site (arrow 3).
The suspension culture helps minimize cell losses that may occur when EBs are dissociated from adhesion cultures.
Interestingly, Y-27632 does not affect PTEN activity in adherent cells (Fig.6A), suggesting that ROCK only or mainly modulates PTEN activity when cells are dissociated from their environmental cues.
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