In a recent study on wheat straw we found that ensiling prior to HTT improved the effect of HTT pretreatment at lower temperatures as measured by released glucose and xylose after enzymatic hydrolysis [ 22].
After searching and analyzing the wheat genome sequences completed recently through WHEAT URGI, we found that each wheat genome only has one BiP gene, indicating that common wheat may have three BiP gene copies.
Within the wheat data, we found members corresponding to every rice TF family suggesting that TF families are conserved between rice and wheat.
"We found significant reductions in iron, zinc and protein in rice and wheat, and we found significant reductions in iron and zinc in soybeans and field peas as well," he said.
While mining of miRNA in wheat transcriptome, we found an abundance of miRNA 172 target sites.
Comparing protein solubility in E. coli bacteria and the cell-free E. coli and wheat germ systems, we found that the wheat germ system produces the highest solubility rate (97%).
In the wheat data set we found members corresponding to every rice TF family suggesting that TF families are conserved between rice and wheat.
Using Affymetrix Wheat Genome Array, we found that ~11% (6560) of total probe sets were responsive to short and prolonged heat stress treatments in wheat.
Consistent with a lifestyle as a wheat pathogen, we find that the Ptr genome encodes fewer pectin-degrading enzymes, including those in the polysaccharide lyase and GH (glycosyl hydrolase) families 28, 78, 88, 95, compared with the dicot pathogens Verticillium dahliae (Klosterman et al. 2011) and F. oxysporum (Table S19) (Ma et al. 2010).
Further, by comparative confocal analysis of nuclei probed to wheat and rye NORs, we found that in the wheat-rye addition line there is a significant decrease in the number of wheat-origin perinucleolar rDNA knobs, corresponding to a diminution of the rDNA heterochromatic fraction of the dominant (wheat) NORs.
