In addition, the freezing and thawing process could also have produced vesicles with surface properties intermediate between cytosolic side-out and cytosolic side-in plasma membrane vesicles, as we earlier reported for wheat plasma membranes [ 41].
AWPM-19 accumulates in wheat plasma membrane during cold acclimation in response to abscisic acid [ 31].
This is a small number of transport proteins, considering the need to transport minerals such as Pi, Fe and SO4-, carbohydrates, folate, amino acids, vitamins and cofactors in and out of the amyloplast [ 26], and it is likely that many more wheat amyloplast membrane transport proteins will be discovered with improved techniques and increased sequence information.
Previously we showed that, in addition to labelling of cell membranes, wheat germ agglutinin (WGA) can efficiently detect very small regions of developing fibrosis (Ainscough et al., 2009).
(C) PC3 cells transfected transiently and stably with GFP-EN2 plasmid DNA were then co-stained with wheat germ agglutinin membrane stain (red), these were imaged and analysed using the confocal microscopy software to show co-localisation (40X magnification), the arrows on the images depict the cell through which the measurement was taken.
The membrane marker wheat germ agglutinin (WGA) was used as an additional membrane stain for select samples, following antibody preparation.
DF performed the hybridisation of wheat probes to BAC membranes.
Expression of the V5/N-term protein was diffuse and did not co-localise with the Golgi/ER/plasma membrane marker wheat germ agglutinin (WGA) and therefore displays a different pattern of cellular expression compared to Norwalk virus [15].
The membrane of wheat root cells depolarized upon introduction of K+, PHE, or both K+ and PHE.
We also probed for remaining plasma membrane using wheat germ agglutinin, and again found <1% of nuclei had any detectable signal.
Therefore, we investigated TNT connectivity in the coculture by staining the plasma membrane with wheat germ agglutinin (WGA) conjugated to Alexa Fluor-594.
