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In this work the effect of carbon and nitrogen levels and initial pH of the wheat extract culture medium of submerged culture of Ganoderma lucidum on the amount, purity and structural features of exopolysaccharides (EPS) were studied.
A 2∗∗4 factorial design was constructed to evaluate the supplementation of wheat extract with corn steep liquor – CSL (10 or 20 g L−1), yeast extract – YE (2 or 5 g L−1), ammonium sulfate – AS (0 or 5 g L−1) and glucose (20 or 40 g L−1).
The steepness of curves did not change remarkably except the curve for ZEN-containing wheat extract.
However, Grondin et al. (2009) reported on the use of a crude wheat extract as a promising alternative to DMSO.
Thus, bacteria-only cultures were supplemented with 0.5% wheat extract (wheat grains autoclaved in water) to facilitate bacterial growth and to provide flocculent material for A. laticollaris CSH.
We were able to grow cultures on bacteria only after the addition of flocculent material in the form of wheat extract.
Similar(43)
In general, variations were slightly higher for the wheat extracts.
Subsequently, we have calculated LOD values for the mycotoxins in maize and wheat extracts.
To obtain a suitable blank material for spiking, the water-treated wheat extracts were pooled prior to spiking.
The values obtained from the recorded sensorgrams were fitted into calibration curves determined in maize and wheat extracts.
To this end, the multi-standard working solution was spiked into blank wheat extracts at six concentration levels.
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