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In fact, were Chad Kroeger ever to look up from his gnarly grunt-rock work-bench he'd realise the cloud of existential angst that's been shadowing him his whole life is actually a flock of circling pigeons desperate for the glory of firing a hot, wet pellet of excremental gut-juice directly into his yowling great gob.
DNA extracted from 50 ml of each culture was centrifuged and the wet pellet transferred to a 1.5 ml Eppendorf.
Microalgae culture was centrifuged (4000g, 10 min) to obtain wet pellet which was mixed in distilled water, methanol and chloroform (4:5:10 v/v) and left overnight on a shaker at moderate speed.
In the case of immobilized cells, the cell wet pellet correspondent to the mentioned OD was gently mixed with 2.8% sodium alginate and after complete homogenization, the mixture was added to 2.0% CaCl2 using sterile needles and a distance needle solution of 4 to 7 cm.
Cells were resuspended in SB, 1 mM PMSF to a total volume equivalent to 4× wet pellet weight.
Lysate pellets were weighed and a 10% wet pellet weight/volume homogenate made in dPBS.
Similar(52)
The wind whipped bursts of wet pellets across the windows.
He tells customers that wet hardwood charcoal can be dried out, but he recommends tossing wet pellets.
Unlike the other litters, though, the wet pellets need to be stirred daily (they dissolve into a sort of damp sawdust).
After colonies were harvested, wet pellets were kept on ice for 15 min, and then resuspended in electroporation buffer containing gene-targeting plasmids.
It essentially involves balling of fine powders on a rotating disk or drum to produce wet pellets and induration on a moving grate to produce fired pellets.
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