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(Wet ice, Haraguchi believes, is a vector for the bacteria that can contaminate raw fish).
In this study, eight participants tested four styles of winter boots on smooth wet ice.
Upon analysis samples were thawed at 37 °C in a water bath for 2 min and then kept on wet ice.
One footwear outsole material and tread combination outperformed the others on wet ice allowing participants to successfully walk on steep longitudinal slopes of 17.5° ± 1.9° (mean ± SD).
The results showed that the estimated probability of a fall was high (over 0.9 for wet ice conditions) when the exit aids were not utilized.
Samples were transported to the laboratory on wet ice.
All steps were conducted at 4°C, on wet ice and with ice-cold solutions.
All samples stored on wet ice were immediately transported under refrigeration to the laboratory for processing.
The samples were centrifuged in the field then transported on wet ice to the laboratory at IEDCR where they were stored at −70°C.
EPC were quantified using the following approach: EDTA collection tubes were transported on wet ice into the laboratory and proceeded within 2 to 3 hours.
Sub-samples of 100 mL were collected for microbiological counts (coliforms and heterotrophic bacteria), placed in sterilised bags and stored at 4°C on wet ice.
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