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The extraction of metals was performed by wet extraction (in HNO3 0.5N) for the soil samples, and by ash digestion (with HNO3 0.5N) for the nettle and snail samples.
Several processing technologies were evaluated: centrifugation and solvent extraction (POS Biosciences), thermochemical conversion (Valicor), hydrothermal liquefaction (PNNL), catalytic hydrothermal gasification (Genifuel), combined heat and power, wet extraction (OpenAlgae), and fermentation.
In this work the methods for wet extraction of algae lipids using traditional organic solvents, supercritical CO2 and CO2 switchable solvents are compared with dry extraction on an energy consumption basis.
Wet extraction transesterification processes, while significantly reducing the drying energy input, typically involve solvent-based extractions that lead to concerns over solvent disposal (Torres et al. 2013).
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Enchytraeids were sampled using six-fold cores (diameter 5.8 cm×15 cm, 6 rings of 2.5 cm height each), extracted using wet funnel extraction, identified, measured and counted.
Wet solvent extraction involves mechanical cell rupture, lipid extraction via solvent contacting, physical phase separation, thermal solvent recovery, and transesterification.
The sediment was separated into allogenic and authigenic fractions using a wet chemical extraction technique.
This research assessed the effect of coagulation, flocculation, and centrifugation on the wet lipid extraction procedure, which fractionated microalgae into hydrolyzed biomass for fermentation into acetone, butanol, and ethanol, an aqueous phase as growth media for genetically engineered Escherichia coli, and a lipid fraction for the production of biodiesel.
A combination of solution-state 31P-NMR, P K-edge XANES and wet chemical extractions was applied.
The activities include wet labs (DNA extraction, PCR, and sequencing), sequence analysis (cleanup, alignment), phylogenetic reconstruction (tree building and molecular clocks), and population genetic analysis (genetic diversity, demographics).
We first evaluated the impact of freeze-drying compared to wet tissue metabolites extraction using NMR and LC MS with a reversed phase liquid chromatography.
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