"wet box" is not correct or usable in written English.
It is not a commonly used phrase/idiom.
After another wash with cold 1 × PBS three times for 5 min, they were treated with normal goat serum for 30 min at RT, followed by the addition of primary antibodies (1 200) and incubation in a wet box for 2 h at RT or 4 °C overnight.
The slide was then incubated at 38 °C in a wet box for 2 × 35 min.
Slices were washed with PBS twice, excess liquid was removed using tissue paper, and 50 μL of TUNEL reaction mixture was added drop-wise, followed by incubation in a wet box at 37°C for 60 min.
After being cooled, the sections were mounted by fetal bovine serum for 60 minutes, and then primary antibody was added and kept in wet box at 4°C for 24 hours.
Prehybridization was performed by adding prehybridizational solution (provided in the CDK4 in situ hybridization kit) on the slides and incubated in wet box at 38°C for 4 h.
They were shocked to see Imelda Marcos's shoes and gowns when they opened the wet boxes, officials said.
Alex Hartov saw something special in the filthy, wet boxes that sat untouched for years in a basement in Hull, Mass .he boxes, damaged by a flood, contained hundreds of old records that belonged to his wife's uncle, who had been the host of a Jewish radio show, "The Yiddish Hour," in Boston in the 1950's.
This weekend it was the Calder that rampaged down the road, the river having burst its banks during the wettest Boxing Day recorded in the West Yorkshire town.
According to the campaign video, around 1,200 businesses were badly hit in the floods in this area of West Yorkshire after the river Calder burst its banks on the wettest Boxing Day on record.
The procedure we followed was based on that employed by Galsworthy et al. [ 41], in which mice must navigate the maze and cross from the wet start box to the dry goal box in order to escape the cold water.
The boy picks barnacles off the bottom of the beached skiff with the point of a jackknife, while the men show the police captain the Frenchman's clothes and flipper, the wet pencil box, the drawings from his room.
