Sentence examples for western transfers from inspiring English sources

Immunofluorescence and western transfers were performed as described previously (Stanton et al., 2010).

SDS-PAGE and Western transfers were carried out essentially by the methods of Laemmli [ 23] and Towbin et al [ 24].

Protein was then transferred to 0.45 µm pore nitrocellulose membranes (Life Technologies) in 1× Western transfer buffer (Life Technologies) at a constant 30 V for 1 h using an XCell II Blot module (Life Technologies).

The Viacom Foundation, successor to Gulf and Western, transferred this interest to the New York City Economic Development Corporation, which is not a municipal agency but a nonprofit corporation operating under contract to the city, with a president and chairman appointed by the mayor.

The gels were then transferred onto PVDF membranes for 7 min using the iBLOT western transfer system (Invitrogen, Carlsbad, CA, USA) at room temperature.

Generation of cell extracts, protein electrophoresis, Western transfer and antibody detection were done using standard procedures.

Western transfer to PVDF membranes used a BioRad Mini-PROTEAN Tetra cell system.

Proteins were separated by Laemmli sodium dodecyl sulfatepolyacrylamide gel electrophoresis and transferred by semidry Western transfer to a nitrocellulose membrane (Bio-Rad).

Separation via 1D PAGE was performed on Invitrogen pre-cast gels with Western transfer onto PVDF membrane using either a Criterion or Invitrogen blotter.

Embryonic day 13.5 hearts were harvested and 50 µg of total cell lysates were resolved by 7% SDS-PAGE followed by western transfer to a nitrocellulose membrane.

Western transfer was performed on a wet transfer apparatus (Bio-Rad).