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We applied Western methodology to assess its effectiveness and safety for primary dysmenorrhoea and to evaluate the compliance and feasibility for a future trial.
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While there is no doubt that research should be conducted according to Western methodologies (i.e., searching for and finding active ingredients before submitting them to laboratory tests and clinical trials), the question of what approach should be taken is still roaring.
Since many Western (see methodology for definition) universities have incorporated text-matching software checks as a part of the submission process, plagiarism has been somewhat deterred, if not always detected (Sivasubramaniam 2013).
Standard western blot methodology was used to visualize proteins.
The results of this study demonstrate a quantitative increase in fibrinogen in neonates (Day 1 and Day 3) compared to adults, a result that was validated using the western blot methodology.
Conditioned media were prepared as described in the western blot methodology.
KLF4 protein levels were examined using the Western immunoblotting methodology reported elsewhere, with β-actin as loading control [ 24].
In addition we describe a list of working antibodies for cell cycle and apoptosis proteins by western blot methodology.
Prior to the application of the protocol based on that described by Narang and Perry [ 23], the same protocol of purification and concentration used for Western blotting methodology was used for SAF visualization.
This method was employed despite the large sample size of the KEEPS trial, making this the largest investigation to utilize quantitative western blot methodology for assessment of these relationships.
The quantitative western blot methodology that we have chosen has been cited as the gold-standard for RBP4 determination [ 18], and utilizes the full-length RBP4 protein and monoclonal antibody specific for full-length RBP4 protein, greatly increasing validity compared to numerous prior investigations utilizing immunoassay.
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