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We found that transplant tumor was not formed in nude mice after cells were vaccinated into subcutaneous tissue of nude mice (data not shown).
Thereafter, we carried out peptide-based immunotherapy against various types of cancer in which Lck-derived peptides were vaccinated into HLA-A24+ or -A2+ cancer patients (Sato et al, 2003; Mine et al, 2004).
More than 95% of the cells expressed CD3. SMSCs (P4) were vaccinated into 96-well plates at a concentration of 1 × 10/ml, in a final volume of 100 μl fresh medium (10% FBS + high-glucose DMEM).
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Five were vaccinated in the BCG negative arm before enrolment into the BCG positive arm began.
Age-matched female BALB/c mice (Harlan, UK), housed in specific-pathogen free environments, were vaccinated with equal amount of vaccines into each legs via the intramuscular route (i.m). using either a heterologous prime-boost viral-vectored regime or protein-in-adjuvant prime-boost regime.
The animals were not screened for pestiviruses before their introduction into the herd but were vaccinated regularly for BVDV.
Subjects were vaccinated at different ages and with different schedules, all of which were taken into consideration.
No wild herds were vaccinated.
Around half were vaccinated against measles.
Prove that they already were vaccinated.
More than 3,300 people were vaccinated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com