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All haplotypes assigned computationally were unambiguous, with statistical probability exceeding 99%.
Alignments of protein coding gene (cyt b) were unambiguous with no insertions or deletions.
Most homeologies were unambiguous, with the exception of the homeologs of ssa1qb and ssa4q which seems to be a fusion of ssa11qb and ssa13qb, and ssa10 for which we could not uniquely separate matching regions on ssa16qa and ssa23.
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But at the same time, the message he delivered in Israel was unambiguous: with the Modi government in power, the days of downplaying India's ties with Israel or hyphenating them with the Palestinians were now a vestige of the past.
The protein alignment is unambiguous with only a single seven residue insertion/deletion difference between human and mouse ORF2p at the beginning of the endo domain.
Yet, expression of the housekeeping gene β-actin was unambiguous, with amplification of both a 460-bp fragment (Figure 3, Lane B) as well as the entire coding sequence (Figure 3, Lane J).
In all cases, alignment was unambiguous with no indels or frameshift mutations.
In practice the vast majority of stimulus investigation was unambiguous with the mouse actively sniffing with snout within a couple of mm of the tea strainer.
For both COI and ITS2 the alignments were unambiguous, the former with no gaps, and the latter with only a few, unambiguously placed gaps.
In addition, the following uniqueness criterion was imposed to make sure that the two best alignments were unambiguous: for a query with three or more alignments, the alignments ranking third or worse were compared with the second best alignment (constituting the "short" partner of the reconstructed transcript).
The alignments of COI, Rhodopsin and Histone H3 were unambiguous, but several indels with multiples of three were observed in TMO-4C4 and RAG1, especially in species of the Tripterygiidae.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com