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Cells were treated the next day with the test compounds in the presence of 1 nM E2.
Cells were treated the next day with tunicamycin (1 μg/ml) and different concentrations (serial dilution) of each compound for 7 hr.
Cells were plated 1 day before drug treatment in a six-well plate at 5 × 10 cells per well for 2D culture, and were treated the next day.
Cells were treated the next day with 100 nM thapsigargin and 10 µM of the library compounds (diversity library of 106,281 compounds) for 6 hr.
While it would not offer an instantaneous result, there could still be significant benefits to patients if they were treated the next day rather than waiting 1 2 weeks as under standard care.
Cells were seeded in 96-well plates (10 × 10/well for adherent cell lines and 50 × 10/well for suspension cell lines) and were treated the next day as described in the figure legends.
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If no ALT was observed, the next three patients were treated at the next activity level.
The women, who were not treated due to a false negative result, were treated at the next occasion following notification of the false negative result.
If none of the three patients experienced dose-limiting toxicity (DLT), the subsequent patients were treated at the next dose level.
In Trial 2, mice bearing ZR-75-1 mammary tumor cells were treated, starting the next day, with the p97-ADR conjugate, SYN002.
If the patient was not present they were treated on the next day.
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CEO of Professional Science Editing for Scientists @ prosciediting.com