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When cells transfected with LMP2A siRNA were treated singly with 5-FU or with the combined drugs (5-FU followed by LY294002), the expression of p-AKT was significantly reduced than that in cells transfected with the scrambled siRNA.
After overnight incubation to allow the cells to attach to the bottoms of the wells, the cells were treated singly with 5-FU (0.1-300 μM) or LY294002 (0.1-80 0.1-80luted in DMSO for 24 h, 48 h, or 72 h, or with a sequential application of the drugs.
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Our Xingjiang samples express clear characters similar to the peoples of Central and Western Asia, and they could be treated singly as a "northwest group".
Mice were treated with PTX and DDP, singly or together (PTX+DDP).
MCF7S cells were treated with vehicle alone or singly with various concentrations of 4-OHT and ICI at the time of seeding.
For these experiments, T-Ag expressing cell lines were treated with LY294002 and U0126 singly or in combination, followed by in vitro invasion and migration analyses using Boyden chambers.
Osteoblast-like cell cultures were treated with PDGF and synthetic peptides, singly and as combinations, and compared to non-treated control cell cultures.
Primary mouse hepatocytes deficient in p53, p21 and/or Rb, singly or in combination were treated with TGFβ for 24 to 96 hours.
In another preclinical study, immunodeficient mice bearing human LNCaP prostate tumours were treated with D and/or surgical castration applied singly, concurrently or in different sequences.
CMV-rtTA TetO-p16 miCMV-rtTA TetO-p16nsgeniCMV-rtTA TetO-p16ittermice controls were treandd with Dox chow at weaning, posinglyl d20.
The advantage of this method is that the effects of multiple factors on the variable of interest are not treated singly but are considered simultaneously.
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