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To investigate the role of DES and the prior treatment with KMnO4 further, pristine graphene flakes were treated directly with some types of DES without prior purification.
Seeds, in seed packets as described above but without the sterilisation step, were treated directly in PVS2 for different times (0, 30, 45 and 60 min), and then transferred into Nunc™ cryovials (Sigma-Aldrich, Gillingham, UK) and covered with PVS2.
Matching controls were treated directly after separation from the colony.
For NLP obtained from body fluids, except indicated otherwise, these fluids were treated directly with the 3 precipitating reagents to a final concentration of 8 mM each.
Checkerboard assays revealed that when activated myoblasts were treated directly with Ang II no increase in migration was observed; however, when exposed to a concentration gradient of Ang II, a significant increase in the number of migrating cells was demonstrated (Figure S4A).
Oily samples were treated directly.
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Many therapists and doctors would also be surprised by the claim that nightmares can be treated directly.
The sodium salt of the alkyne formed in this reaction is not normally isolated but is treated directly with an alkyl halide.
As for relatively low acid concentration wastewater FA1, it was treated directly by RO technique.
To prove this, an experiment was conducted in which pristine graphene was treated directly with DES 5.
Innovation milieu models cannot be treated directly as the development of third-generation innovation models since they shift from the meso to the micro level.
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