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To test this possibility, CD4loCD8lo thymocytes from Rec-HY Bim-deficient male mice were transfered into in vitro systems of T cell development.
The DNA was injected into fertilized eggs of C57BL/6J×SJL/J mice, and eggs were transfered into foster mothers, all at the University of Miami Transgenic Facility.
At the age of 5 days, 10 females per vial and 10 vials per genotype were transfered into media for fasting (0.8% agarose w/v in PBS).
Subsequently, the extracts were transfered into buffer including 8 M urea and finally into the complete assay buffer including all of the former plus 10 mM DTT (Unfolding-activation buffer: 10 mM TrisHCl (pH 8.0), 8 M urea, 10 mM DTT).
primers were transfered into a 25 µl PCR reaction containing 0.2 µl cDNA, 1 U (PeqGold Taq-DNA Polymerase (Peqlab, Erlangen, Germany) 2.5 mM dNTP mix, 50 mM MgCl2 and 10 pM/µl of each sense and anti-sense nested neomycin resist.
Immediately after mixing the materials were transfered into a cylindrical teflon mould (10 × 1.5 mm).
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Samples were transferred into the microwells.
All three were transferred into the care of immigration officials.
After washing, the cells were transferred into scintillation vials.
The samples were transferred into a 15 ml centrifuge tube.
Pre-germinated seeds were transferred into laboratory vases.
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